Fluorescent Probes: Illuminating the Secrets of Molecular Mechanisms
Fluorescent probes are molecules that can absorb light of one wavelength and emit light of a different wavelength, a process known as fluorescence. This property makes them incredibly useful for a wide range of applications, including molecular imaging, biosensing, drug discovery, and environmental monitoring.
Topics Covered:
The basics of fluorescence
Explore the applications of fluorescent probes
Understand the mechanism of fluorescence and
Discuss the challenges and advancements in the field
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. When a fluorophore absorbs light, it is excited to a higher energy state. When the fluorophore returns to its ground state, it emits light at a longer wavelength than the absorbed light. This difference in wavelength is known as the Stokes shift.
Mechanism of Fluorescence
The mechanism of fluorescence can be explained using the Jablonski diagram. The Jablonski diagram shows the different energy levels of a fluorophore and the different ways in which it can transition between these energy levels.
When a fluorophore absorbs light, it is excited to a higher energy state (S1). The fluorophore can then return to its ground state (S0) by emitting a photon of light. This is known as radiative decay. Alternatively, the fluorophore can return to its ground state by losing energy as heat. This is known as non-radiative decay.
The quantum yield of a fluorophore is a measure of the efficiency of fluorescence. The quantum yield is defined as the number of photons emitted per photon absorbed.
There are many different types of fluorophores, each with its own unique properties. Some common types of fluorophores include:
• Organic dyes
• Quantum dots
• Fluorescent proteins
• Upconversion nanoparticles
• Semiconductor nanocrystals
Timestamp:
00:00 Introduction to Fluorescent Probe
00:06 Topics Covered
00:35 What is fluorescence
00:54 Historical Perspective
01:14 Mechanism of fluorescence
01:58 Fluorophores
02:13 Properties of Ideal fluorophore
02:26 Applications
03:18 Imaging Techniques
04:02 Biosensors
04:41 Drug Discovery
04:53 Environmetal Monitoring
05:09 DNA Sequencing
05:32 Challenges
06:20 Advancements
06:39 Impacts
07:18 Future Directions
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