the bumbling biochemist

The basic science behind bubbles

NucleoTides vs nucleoSides

The biochemistry behind hair frizz & curls: hydrogen bonds, disulfide bonds, & intermolecular forces

Tips for giving a research presentation (especially to a more general audience)

Enzyme inhibition: types (competitive, noncompetitive, uncompetitive, mixed, covalent) & evidence

SDS-PAGE calculations - walk-through of how to calculate and prepare to load equal masses per lane

Peptide bonds and secondary protein structure

Hemoglobin regulation – Quick review/overview of effects of oxygen, 2,3-BPG, CO, Bohr effect, etc

pH calcs Part. 1: Intro to question types, strategies, and equations

Adobe Illustrator tips: transform each (not transform) for formatting axes labels etc. + PDF tips

Preparing for professor-ing

Where ATP's metabolic power comes from and how it's used

Metabolism study strategy suggestion - integrated chart spreadsheet (template link in comments)

Practical Python Practice Problems for People who Pipet

Biochemistry of blood sugar regulation & glucose homeostasis

Nonfiction science book recommendations

Python enumerate - a super helpful function to know

Logic of glycolysis & gluconeogenesis - Part I

Biochemical binding thermodynamics - Kd, Ka, and their interpretation

Bacterial growth media (LB, TB, SOB, SOC, etc.)

Structural strategies for getting a look at macromolecular complexes & the meaning of "apo"

Useful Adobe Illustrator shortcuts/hot keys (a guide to my favorites)

pandas tips for getting a quick overview of a dataframe (head, head.T(), info, describe, etc.)

EZ vision sample loading dye (not a paid endorsement, I just find it helpful)

Kinases vs Phosphatases & their roles in glycolysis and gluconeogenesis

Enzyme kinetics fundamentals & terms: Km, kcat, specificity constant/catalytic efficiency, activity

NAD+ regeneration - anaerobic glycolysis/fermentation & the Cori cycle

Hemoglobin pt 1: allostery cooperative binding

Steady state vs equilibrium demo test

One year update

Salvaging mis-measured solutions. Adjusting mass &/or volume to still get the desired concentration

Critically evaluating supplements, etc.: systematic reviews, meta-analyses, biases, evidence, etc.

Interpretting co-IP & other pulldown figures

Protein "mutant" terms & nomenclature - how we not technically-correctly talk about altered proteins

Save yourself a lot of struggle - start with stock solutions not solids!

HEPES - the when, why, & how of using this pH buffer

Solid state oligonucleotide synthesis (phosphoramidite method)

Practical advice for increasing your PCR product yield

UniProt entries: a guide to using UniProt as a launch pad for learning about a protein of interest

G-Protein Coupled Receptors (β-adrenergic, etc.)

Size Exclusion Chromatography (SEC) (aka Gel Filtration): preparative & analytical on the AKTA

Some advice for college graduates (past, present, & future) & thoughts on communicating knowledge

“Dual enrollment” - you can take community college courses in high school (at least in the US)

The gist of group meetings (the science lab kind)

Practical tips for using a vacuum manifold for minipreps and similar spin columns

Here’s to getting goopy! A quick geek out about what I love in the lab

"Oligos", oligo- & poly-

Casting/pouring agarose gels - what to do and what goes on when you do so!

Biochemical basis of the Bohr effect - and other ways Hemoglobin O2-binding is affected (by CO etc.)

Pipetting viscous liquids - reverse pipetting, etc.

Mendeley and other reference management tools + tips for finding & managing the correct references!