Practical tips for using a vacuum manifold for minipreps and similar spin columns
If you have a lot of mini preps (or other nucleic acid purification columns) to run and/or you have larger volumes than a single column can accommodate in one spin, the vacuum manifold can give you a win! Here are a couple tips to help ensure that the vacuum sucks but your results don’t!
make sure all of the spots not in use are stoppered
turn off the vacuum (and/or turn the stopcock to horizontal) once the liquid has all been pulled through - don’t leave it sucking the dry membrane or it can damage it
after you do what you need to do on the vacuum, spin it (in waste tube) to ensure it’s dry (remember any liquid on the sides of the tube won’t get removed by the vacuum!)
use a repeater pipet to make the washes faster, BUT
avoid splashing on the sides of the tube while adding the wash buffer
label the columns on the ledge-y part so you see it when it’s in the tube & make sure the wash buffer doesn’t wash off the label
don’t worry about the vacuum manifold brand matching the column brand - they typically all have the same closures so you can use them with whatever
try not to loose the stopper, but if you do, a 15mL falcon tube can save the day…
more on mini preps: http://bit.ly/minipreps ; YouTube: • Minipreps (alkaline lysis plasmid pur... s
more on nucleic acid spin columns: http://bit.ly/spincolumns & • Spin column nucleic acid purification...
more practical lab tips: https://bit.ly/lab_tricks_page & • Practical lab tips & tricks
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com
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