Coomassie Purity Staining of Recombinant Antibodies - Addgene Protocol
Looking for a simple, fast, and cheap way to check an antibody’s purity? Well, you’ve found it, with Addgene’s protocol for Coomassie Purity Staining of Recombinant Antibodies! Join Amrita Rhoads, a Lab Tech II at Addgene, as she walks you through this simple, time-saving, in-expensive process.
For the full written protocol, head to https://www.addgene.org/protocols/coo...
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00:00 - Intro
01:04 - Materials & Reagents
02:25 - Preparation
03:49 - SDS Page
06:41 - Staining the Gel
08:26 - Image Analysis
12:37 - Outro
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Featuring: Amrita Rhoads and Ashley Waldron
Written by: Rachel Leeson & Quintin Marcelino (based on a protocol by Angela Holmes)
Directed by: Quintin Marcelino
Assistant Director: Anna Kamens
Designs by: Jason Snair
Edited by: Anna Kamens & Quintin Marcelino
Animations by: Quintin Marcelino
Special Appearance by: Webster Kiwi Kitty
Music and Sound Effects courtesy of Epidemic Sound
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