Recombinant Antibody Affinity Purification with Protein A or Protein G Protocol
Do you really know what's in your protein supernatant? If not, it sounds like you’re looking to do some Antibody Purification! Purification lets you isolate the antibodies from the other proteins in the supernatant, leaving you an accurate measurements and sparkling clean calculations. Join Amrita Rhoads, a Lab Tech at Addgene, as she shows you just how easy it is to become a purifying pro!
For the written protocol, head to: https://www.addgene.org/protocols/aff...
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Intro - 00:00
Materials - 01:46
Transfection Info - 03:07
Prep - 03:28
Affinity Chromatography - 03:56
Buffer Exchange - 07:12
Storage - 10:11
Next Steps - 10:33
Outro - 10:52
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Credits:
Featuring: Amrita Rhoads
Written By: Rachel Leeson, & Quintin Marcelino
Directed, Animated, & Edited by: Quintin Marcelino
Videography by: Quintin Marcelino
Sound Design by: Quintin Marcelino
Designs by: Jason Snair
Additional Designs by: Quintin Marcelino
Music and Sound Effects courtesy of Epidemic Sound.
Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational purposes only. If you choose to reuse or repurpose this SOP in another location, please note that you do so at your own risk; you should ensure that any local guidance is also adhered to. None of the authors, contributors, administrators, or anyone else associated with Addgene, can be held responsible for your use of the information contained in or linked to from these web pages.
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