Here’s to getting goopy! A quick geek out about what I love in the lab

Enzyme inhibition: types (competitive, noncompetitive, uncompetitive, mixed, covalent) & evidence

Hemoglobin pt 1: allostery cooperative binding

Enzyme kinetics fundamentals & terms: Km, kcat, specificity constant/catalytic efficiency, activity

Peptide bonds and secondary protein structure

Hemoglobin regulation – Quick review/overview of effects of oxygen, 2,3-BPG, CO, Bohr effect, etc

Tertiary & quaternary protein structure

Tips for giving a research presentation (especially to a more general audience)

3 things to check each time you pipet (with a micropipet)

Adobe Illustrator tips: transform each (not transform) for formatting axes labels etc. + PDF tips

Salvaging mis-measured solutions. Adjusting mass &/or volume to still get the desired concentration

One year update

Critically evaluating supplements, etc.: systematic reviews, meta-analyses, biases, evidence, etc.

NAD+ regeneration - anaerobic glycolysis/fermentation & the Cori cycle

Where ATP's metabolic power comes from and how it's used

Metabolism study strategy suggestion - integrated chart spreadsheet (template link in comments)

Kinases vs Phosphatases & their roles in glycolysis and gluconeogenesis

Logic of glycolysis & gluconeogenesis - Part I

Steady state vs equilibrium demo test

Membrane transport biochemistry - gradients, potential, & active vs passive transport

Interpretting co-IP & other pulldown figures

Biochemical binding thermodynamics - Kd, Ka, and their interpretation

Preparing for professor-ing

pH calcs Part. 1: Intro to question types, strategies, and equations

SDS-PAGE calculations - walk-through of how to calculate and prepare to load equal masses per lane