Automated Two-Step 2D Protein Purification with Collection in a Sample Loop for Re-Injection

Published: 29 April 2015
on channel: Bio-Rad Laboratories
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This video provides an overview of how preparative chromatography systems may perform sequential tandem loading and elution of select fractions across 2 columns via an intermediate sample loop.

This tandem purification method can be applied to your research and reduce hands-on time. This tandem purification method is available as a software template in ChromLab™ software, the integrated software package for the NGC™ chromatography system. This template is one of three new tandem purification templates. These templates may be edited to fit specific user requirements.

ChromLab software controls all functions for laboratory-scale protein purification including instrument setup and calibration, method development, real-time monitoring and system control, chromatogram comparison, and peak analysis.

ChromLab software streamlines your workflow with multiple templates for common chromatography methods and for complete multistep tandem chromatography for maximum versatility. Select from our column database and the software will automatically adjust flow rate and pressure parameters optimal for that column to prevent column damage. Alternatively, create your own custom column database for maximum flexibility.

The flexible, modular, and economical design of the NGC system makes it the instrument of choice for method development and scale-up. NGC systems are available in six preplumbed, factory-tested configurations with two different flow rates. Each preconfigured system can be further customized and upgraded by adding valves, detectors, or pumps to meet specific application needs. Any system can be configured for operation at either high or low flow rate by simply changing the pump head on the system pump module. With the NGC system, a single hardware platform can be modified as needed to suit changing applications and scales.


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