Did You Know that Beyond Sensitivity, ddPCR Provides Unrivaled Precision and Accuracy?

Published: 21 July 2023
on channel: Bio-Rad Laboratories
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Three of the main advantages of digital PCR (dPCR) are absolute quantification, sensitivity, accuracy, and precision. However, choosing the right dPCR system is important to get accuracy and precision in your results.

Methods that are highly accurate and precise provide the right answer with consistency. For dPCR Poisson statistics dictate that the accuracy and precision of results are dependent on the accuracy and precision of sample partitions. This means that to get reliable dPCR results you need to know your partition volume accurately and that your partition volumes need to be consistent.

Only Bio-Rad Droplet Digital™ PCR (ddPCR™) partitions samples using droplet technology. Samples are partitioned into droplets using time-tested systems and in-instrument quality control systems look for and reject rare occurrences of droplets that are either too small or too large. In addition to ddPCR technology having more than 12 years of proven results, the technology has been validated by Australia’s National Measurement Institute (NMI), which has used both optical profilometry and nucleic acid based methods to validate ddPCR droplet volumes with a precision of 14 picoliters.

Only Droplet Digital PCR delivers the greatest precision, accuracy, and sensitivity. So, ask yourself, can your digital PCR provider tell you the accuracy and precision of their partitions and the results you’ll get? Learn more: https://info.bio-rad.com/what-differe...

#digitalPCR #ddPCR #dPCR #accuracy #precision #partition

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