Direct and indirect flow cytometry video protocol
Flow cytometry is a popular laser-based technology to analyze the characteristics of cells or particles. Watch our flow cytometry video protocol that teaches you how to prepare and stain your cells for flow cytometry analysis.
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Timestamps:
00:00 Introduction
00:13 What you will need
00:41Titration (optional)
01:00 Preparing a primary antibody solution
01:15 Re-suspending cells and measuring cell viability
01:48 Centrifugation
02:03 Blocking step
02:37 Staining with primary antibody
03:51 Secondary antibody step (optional)
04:55 Checking for live/dead cells
05:16 Storing the cell suspension
Watch video Direct and indirect flow cytometry video protocol online without registration, duration hours minute second in high quality. This video was added by user Abcam 16 February 2021, don't forget to share it with your friends and acquaintances, it has been viewed on our site 6,382 once and liked it 58 people.