Intracellular Ca2+ was visualized with fluo-4. T0 = 25°C, ΔT = 12°C (left, a polar bleb). T0 = 37°C, ΔT = 13°C (middle, multiple blebs). T0 = 37°C, ΔT = 19°C (right, "motionless"). Scale bar represents 10 µm. See the article by Oyama et al. in the July 21 issue of Biophysical Journal.
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