Tissue engineering 3-D cultures
Three-dimensional (3-D) culture can more accurately replicate the natural growth environment for many cells compared to 2-D monolayers. The extracellular matrix (ECM) plays an important role in promoting cell proliferation, differentiation, and complex cell-matrix interactions, including these effects in early in vitro stages of drug screening could yield results more predictive of in vivo assays. In our approach, we generate cell-laden hydrogel beads and incorporate elements of the ECM, including collagen and reconstituted basement membrane matrix, to provide cues from the natural microenvironment.
The video demonstrates second harmonic generation imaging shows collagen distribution within the core-shell beads. Optical sections were acquired at 5 µm intervals from the top of the bead to 300 µm into the bead.
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