Cell Culture: Aseptic/Sterile Technique Dos & Don’ts | CST Tech Tips
Contamination by microorganisms in your cell lines can disrupt your experiments. Even if you’ve been trained for cell culture in your lab, it’s a good idea to review your sterile tissue culture technique from time to time. This video demonstrates the “Dos & Dont’s” of proper sterile cell culture, which can keep your precious cell and tissue cultures free of contamination.
Contents:
0:00 Introduction
0:37 The biosafety cabinet
1:09 Before you start
1:48 Cell culture best practices
2:22 Organizing your workspace
2:50 Pipetting and handling liquid in the biosafety cabinet
3:35 Other tips for sterile cell culture
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