Reinvent how you teach DNA Cloning | Citizen Science
Citizen Science is a template of scientific participation that can be co-opted by teachers to engage large numbers of students in research training. On this channel we talk about science education, laboratory skills, and professional learning for teachers and all of these themes will converge over the next few videos in ideas for lab classes that promote inquiry-based learning through Citizen Science. Today we will start with recombinant DNA technology or molecular cloning, which is a common point of entry into inquiry-based lab classes. Students design PCR primers targeting a gene of interest, prepare the PCR reactions, setup restriction digest and ligation experiments, and verify the cloning of recombinant plasmids through gel electrophoresis and DNA sequence analysis. The sequential nature of these experiments highlights the importance of controls at each step, providing students with a system where troubleshooting and optimisation are vital for success.
The blog post accompanying this video: jackwang.com.au/blog/stolenmoments1
My paper on Course-based Undergraduate Research in Molecular Biology: https://pubmed.ncbi.nlm.nih.gov/28859...
#biolabcollective #citizenscience #dna #cloning #teaching
Related videos:
PCR: • Polymerase Chain Reaction (PCR) & Gel...
DNA transformation: • How to transform DNA
Colony PCR: • Colony PCR: save time in DNA Cloning
DNA Sequencing: • DNA sequencing - Expectation vs Reality?
0:00 Introduction
1:00 Course-based Undergraduate Research Experiences (CUREs)
1:49 TLDW (Too Long Didn't Watch)
2:51 PCR primer Design
4:15 Restriction Digests
5:00 DNA ligation & Transformation
5:35 Confirming positive clones
6:32 Connecting CUREs to literature and Citizen Science
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