IsoView: Whole-brain functional imaging in larval zebrafish
IsoView whole-brain functional imaging in larval zebrafish.
Dorsoventral (left) and rotating (right) maximum-intensity projections of a multi-view deconvolved IsoView time-lapse recording of the brain of a 3-day old larval zebrafish expressing the calcium indicator GCaMP6s throughout its nervous system. Functional imaging was performed at 1 Hz. The video captures several instances of motor-related, large-scale brain activity in the awake animal. The color code shows low and high fluorescence intensity levels in blue and green, respectively. (Video 5).
Within less than a second, the new IsoView microscope produces images of entire organisms, such as a zebrafish or fruit fly embryo, with enough resolution in all three dimensions that each cell appears as a distinct structure.
Video: Keller Lab, HHMI/Janelia Research Campus
News story: https://www.hhmi.org/news/seeing-big-...
Visit the Keller Lab at Janelia: https://www.janelia.org/lab/keller-lab
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