IsoView: Whole-animal functional imaging in larval Drosophila
This video shows projections of three-dimensional IsoView time-lapse image data of an entire Drosophila larva expressing a fluorescent indicator of neural activity through the nervous system, including the brain, ventral nerve cord (the equivalent of the vertebrate spinal cord) and the peripheral nervous system. (Video 3)
Within less than a second, the new IsoView microscope produces images of entire organisms, such as a zebrafish or fruit fly embryo, with enough resolution in all three dimensions that each cell appears as a distinct structure.
IsoView microscopy was developed by Philipp Keller and colleagues at the Howard Hughes Medical Institute's Janelia Research Campus.
IsoView microscopy captures the entire animal in 500-millisecond intervals with isotropic spatial resolution. Unlike unlike conventional microscopy, resolution in IsoView is high in all three dimensions.
This combination of high imaging speed and high resolution in an entire behaving animal is demonstrated in particular in the rotating view to the right, which shows that IsoView images are crisp and of high quality irrespective of the angle under which the data is viewed.
The color code shows low and high fluorescence intensity levels in blue and green, respectively.
Video: Keller Lab, HHMI/Janelia Research Campus
News story: https://www.hhmi.org/news/seeing-big-...
Visit the Keller Lab at Janelia: https://www.janelia.org/lab/keller-lab
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