Heat-Shock Transformation Protocol (for Bacteria)

Published: 19 April 2021
on channel: Henrik's Lab

65,505

1.2k

Hey Friends,

the Heat-Shock Transformation protocol for chemically competent cells after Calicum Chloride (CaCl2) treatment is a method to introduce genes into bacteria. A short heat shock makes it able for the cells to take up plasmid DNA from the environment.

Transformation, Conjugation and Transduction:
• Transformation, Transduction and Conj...

References:
https://www.ncbi.nlm.nih.gov/pmc/arti...
https://www.ncbi.nlm.nih.gov/pmc/arti...

Thanks
Henrik

Time Codes:
0:00 Introduction
0:59 Transformation in the lab
2:57 Plating bacteria
3:26 Outro

Watch video Heat-Shock Transformation Protocol (for Bacteria) online without registration, duration hours minute second in high quality. This video was added by user Henrik's Lab 19 April 2021, don't forget to share it with your friends and acquaintances, it has been viewed on our site 65,505 once and liked it 1.2 thousand people.

1,276