Mitochondrial Mass & Membrane Potential: Hematopoietic Stem Cells & T-cells l Protocol Preview

Published: 09 August 2022
on channel: JoVE (Journal of Visualized Experiments)
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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry - a 2 minute Preview of the Experimental Protocol

Mukul Girotra, Anne-Christine Thierry, Alexandre Harari, George Coukos, Olaia Naveiras, Nicola Vannini
University of Lausanne, Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne; Swiss Federal Institute of Technology Lausanne (EPFL), Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences; Centre Hospitalier Universitaire Vaudois, Center of Experimental Therapeutics, Department of Oncology; Centre Hospitalier Universitaire Vaudois (CHUV), Hematology Service, Department of Oncology;

Here we describe a reliable method to measure mitochondrial mass and membrane potential in ex vivo cultured hematopoietic stem cells and T cells.

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