DNA Replication in Prokaryotes : Process and Enzyme Involved in Replication
This video explains about DNA Replication in Prokaryotes : Process and Enzyme Involved in Replication
DNA unwinds at the origin of replication.
Helicase opens up the DNA-forming replication forks; these are extended in both directions.
Single-strand binding proteins coat the DNA around the replication fork to prevent rewinding of the DNA.
Topoisomerase binds at the region ahead of the replication fork to prevent supercoiling (over-winding).
Primase synthesizes RNA primers complementary to the DNA strand.
DNA polymerase III starts adding nucleotides to the 3′-OH (sugar) end of the primer.
Elongation of both the lagging and the leading strand continues.
RNA primers are removed and gaps are filled with DNA by DNA pol I.
The gaps between the DNA fragments are sealed by DNA ligase.
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