Western Blot / Protein Immunoblot explained

Published: 08 February 2021
on channel: Henrik's Lab

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Hey Friends,

Western Blot is a technique to determine whether a specific protein is present in a sample. Before, the proteins were separated according to their Molecular Weight using Polyacrylamide Gel Electrophoresis.

Short video about SDS-PAGE:
• SDS-PAGE explained - Protein Separati...

Western Blot allows to transfer the proteins from that gel onto a membrane. This membrane is then incubated with antibodies to detect the presence of the protein of desire.

Very cool sheet about Western Blot:
https://precisionbiosystems.com/weste...

Thanks for watching!
Henrik

Chapters:
0:00 Introduction
0:22 SDS PAGE
0:47 Gel after SDS PAGE
1:25 Western Blot
4:12 Outro

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