Primer Design: Important Considerations and Tips for Good Primer Design
Running a PCR experiment and need to design primers? We all have been there. Primer design is essential for successful PCR, but where do you start? In this video, we’ll go through important considerations and tips for good primer design!
Here are some helpful time stamps for our Primer Design video:
0:41 Primer Length
1:06 Considerations for Melting Temperature (Tm) and Annealing Temperature (Ta)
2:40 GC Content
3:16 Avoid Repeats in Your Primer Sequence
3:39 Avoid Complementary Sequences Within the Primer
4:06 Avoid Secondary Structures in Target Sequences
4:31 Summary
5:07 Avoid List
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