Creating Bacterial Glycerol Stocks
Racheal, a Lab Tech here at Addgene, shows you how to create a glycerol stock solution to store your plasmids indefinitely.
For the full protocol text, visit http://www.addgene.org/protocols/crea...
Bacterial glycerol stocks are important for long-term storage of plasmids. The addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria can be stored stably at -80°C for many years.
This protocol begins after you’ve already created a bacterial culture, so please see out video on Inoculating Liquid Bacterial Culture.
http://www.addgene.org/protocols/inoc...
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0:00 Intro & Overview
1:22 Materials
2:27 Creating the Bacterial Glycerol Stocks
3:39 Recovering Bacteria from your Glycerol Stocks
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Featuring: Racheal Komuhendo
Written, Directed, & Edited by: Quintin Marcelino
Music by: Lauren Duski, Vibe Mountain, and RKVC
Watch video Creating Bacterial Glycerol Stocks online without registration, duration hours minute second in high quality. This video was added by user Addgene 19 February 2020, don't forget to share it with your friends and acquaintances, it has been viewed on our site 73,521 once and liked it 926 people.