How to Sequence a Human Genome in 7 'Easy' Steps!
Join NHGRI researchers at the NIH Intramural Sequencing Center (NISC) as they show you step-by-step how to extract human DNA, sequence it, and interpret the data -- with some fun DNA high jinks along the way.
Step 1: Find a DNA sample.
Step 2: Isolate the cells by centrifugation.
Step 3: Extract the DNA from the sample.
Step 4: Load the sample into the sequencer.
Step 5: Wait for the data to be generated.
Step 6: Use the data you have collected to build the genome.
Step 7: Analyze the DNA sequence data for quality.
Done! High five your team!
#t2t
Learn more about how scientists completed the first complete, gapless sequence of a human genome: https://www.genome.gov/T2T
More about The NIH Intramural Sequencing Center (NISC) https://www.nisc.nih.gov/
*Note: Methods have been oversimplified for communications purposes. Sequencing a full human genome is actually quite challenging!*
Credits:
Producer/Writer/Director: Jenny Montooth, M.A., NHGRI
Videographer/Animator/Editor: Ernesto Del Aguila III, NHGRI
Cameraperson: Darryl Leja, M.A., NHGRI
Audio: Alvaro Encinas, NHGRI
Executive Producer: Sarah Bates, M.A., M.S., NHGRI
Starring: Adam Phillippy, Ph.D., NHGRI
Shelise Brooks, NISC
Sergey Koren, Ph.D., NHGRI
Arang Rhie, Ph.D., NHGRI
Brandon Pickett, Ph.D., NHGRI
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