Pull-Down Assay Protocol
Protein interactions reveal a lot about how proteins and cells function under different conditions. One tool that allows us to look at direct protein interactions is called a pull-down assay. A pull down assay utilizes a bait protein bound to beads in a column to catch protein binding partners. This technique can be used to verify a predicted protein interaction via Western blot or identify novel protein interactions using a total protein stain.
This video is part of the Research Integrated Science Education (RISE) Program that is funded by SENS Research Foundation and Dalio Philanthropies. The program is designed to introduce high school students to aging research and laboratory concepts, such as experimental design and data interpretation, within core curriculum standards. To learn more about RISE and to access additional functionalities such as the transcript glossary function, visit https://www.sens.org/rise
Watch video Pull-Down Assay Protocol online without registration, duration hours minute second in high quality. This video was added by user SENS Research Foundation 25 November 2020, don't forget to share it with your friends and acquaintances, it has been viewed on our site 34,788 once and liked it 896 people.