SDS-PAGE explained - Protein Separation Technique

Published: 01 February 2021
on channel: Henrik's Lab

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Hey Friends,

SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) is used to separate proteins in a sample according to their molecular weight.
SDS binds to linearised proteins and masks the native charge. 1.4 g SDS bind per 1g protein, so that all proteins have a nearly similar mass-to-charge ratio.

After an SDS-PAGE, the gel is frequently used for Western Blot analysis to confirm the presence of a specific protein of interest.

Western Blot:
• Western Blot / Protein Immunoblot exp...

Here is a helpful explanation on SDS-PAGE:
https://ruo.mbl.co.jp/bio/e/support/m...

Thanks & Bye
Henrik

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