How to determine the Protein Concentration with the Bradford Assay
Hey Friends,
protein quantification techniques are used to determine the total concentration of protein in a solution. One among many colorimetric assays for this is the Bradford Assay developed by Marion Bradford. The assay is easy to perform and delivers results in a short time. The Bradford test is based on Coomassie Brilliant Blue G250, a dye which binds to proteins in an acidic environment.
References:
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254. https://www.sciencedirect.com/science...
https://www.bio-rad.com/featured/en/b...
https://www.thermofisher.com/de/de/ho...
Thanks!
Henrik
Time Codes:
0:00 Introduction
1:04 Coomassie Brilliant Blue
1:44 Protein Concentration Determination
2:58 Advantages/Disadvantages
4:02 Outro
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