Inoculating Liquid Bacterial Culture

Published: 09 October 2018
on channel: Addgene

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Inoculation is the process by which you introduce bacteria into liquid media. In this video we will demonstrate best practices to perform inoculation, ensuring sufficient numbers of bacteria for plasmid DNA isolation.

For the full written Inoculations protocol, visit:
https://www.addgene.org/protocols/ino...

For our DNA Isolation Protocol, visit:
https://www.addgene.org/protocols/pur...

For our Creating Bacterial Glycerol Stocks protocol, visit:
https://www.addgene.org/protocols/cre...
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0:00 Intro
0:48 Materials
1:29 Setup
2:34 Inoculation
4:34 Incubation
5:34 Post-Incubation
5:43 Troubleshooting

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