RNA Extraction by TRIzol®

Published: 06 January 2011
on channel: Abnova
267,387
2.1k

( http://www.abnova.com ) - TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol. More videos at Abnova ( http://www.abnova.com ).


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